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. 2019 Mar 19;10:501. doi: 10.3389/fimmu.2019.00501

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Copyright © 2019 Duan, Chen, Razavi, Wei, Tao, Rao and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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α_2BAR overexpression enhances in vitro neutrophils migration. Neutrophils were isolated from α2BAR-Tg and Wt bone marrows and stained with V440 (blue) and CFSE (green), respectively. Labeled cells were mixed at 1:1 ration and used for the Transwell® migration assay in response to MIP2. (A) Upper panel, Representative fluorescent microscopic image showing α2BAR-Tg (Blue) and Wt (Green) neutrophils migrating through the membrane. Lower panel, quantitative analysis showing the migration of α2BAR-Tg and Wt neutrophils. (B) Representative dot plot (upper panel) and quantitative analysis (lower panel) showing the percentages of V440-labeled cells (α2BAR-Tg) and CFSE-labeled cells (Wt) in the bottom well of the Transwell® plate. (C) The Cxcr2 expressions on bone marrows neutrophil with or without treatment of MIP-2 or MIP-2 plus α2BAR agonist clonidine hydrochloride were analyzed by flow cytometry. Data shown are representative of 3 independent experiments. **p < 0.01, ***p < 0.001.