Table 1.
The differentially expressed proteins identified in peripheral blood mononuclear cells (PBMCs) from children with autism and healthy controls.
| No. | Protein name | Accession # | Gene name | Peptides (95%) | % Cov | Fold changec | Pval (FDR correction) |
|---|---|---|---|---|---|---|---|
| 1 | 60 kDa heat shock protein, mitochondrial (+)a, b | P10809 | HSPD1 | 59 | 68.9 | 4.67 | 0.013902986 |
| 2 | 78 kDa glucose-regulated protein (+) | P11021 | HSPA5 | 49 | 64.8 | 2.08 | 2.24E-06 |
| 3 | Aconitate hydratase, mitochondrial (+) | Q99798 | ACO2 | 14 | 42.3 | 3.95 | 9.25E-10 |
| 4 | Alpha-1-antitrypsin* (+) | P01009 | SERPINA1 | 15 | 58.1 | 2.53 | 0.002748253 |
| 5 | Annexin A11 (−) | P50995 | ANXA11 | 18 | 30.5 | 0.55 | 2.69E-07 |
| 6 | Apolipoprotein A-I (+) | P02647 | APOA1 | 15 | 56.6 | 3.78 | 0.000365353 |
| 7 | Aspartate aminotransferase, mitochondrial (+) | P00505 | GOT2 | 7 | 40 | 2.38 | 3.80E-06 |
| 8 | ATP synthase subunit alpha, mitochondrial (+) | P25705 | ATP5A1 | 61 | 65.6 | 2.94 | 0.019869459 |
| 9 | ATP synthase subunit beta, mitochondrial (+) | P06576 | ATP5B | 67 | 83.4 | 4.66 | 5.11E-05 |
| 10 | Calreticulin* (+) | P27797 | CALR | 26 | 58 | 2.93 | 8.09E-06 |
| 11 | Chromobox protein homolog 3 (+) | Q13185 | CBX3 | 10 | 50.8 | 3.31 | 3.02E-05 |
| 12 | Complement C3* (+) | P01024 | C3 | 38 | 43.8 | 2.69 | 1.46E-05 |
| 13 | Cytochrome b-c1 complex subunit 2, mitochondrial (−) | P22695 | UQCRC2 | 26 | 64 | 0.52 | 0.002836642 |
| 14 | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (+) | Q9Y3Z3 | SAMHD1 | 14 | 35.1 | 2.16 | 6.94621E-05 |
| 15 | Dihydrolipoyl dehydrogenase, mitochondrial (+) | P09622 | DLD | 12 | 48.5 | 2.17 | 0.000101238 |
| 16 | Electron transfer flavoprotein subunit alpha, mitochondrial (+) | P13804 | ETFA | 7 | 49 | 2.49 | 4.02001E-08 |
| 17 | Endoplasmin (+) | P14625 | HSP90B1 | 31 | 54.9 | 1.97 | 0.01778616 |
| 18 | Erythrocyte band 7 integral membrane protein (−) | P27105 | STOM | 78 | 77.4 | 0.48 | 2.63E-07 |
| 19 | Flotillin-1 (−) | O75955 | FLOT1 | 20 | 61.4 | 0.45 | 0.003135849 |
| 20 | Flotillin-2 (−) | Q14254 | FLOT2 | 17 | 55.6 | 0.30 | 1.13E-04 |
| 21 | Glutamate dehydrogenase 1, mitochondrial (+) | P00367 | GLUD1 | 17 | 44.6 | 2.50 | 5.48E-05 |
| 22 | Glycerol-3-phosphate dehydrogenase, mitochondrial (−) | P43304 | GPD2 | 34 | 61.1 | 0.58 | 0.001016776 |
| 23 | Heparin cofactor 2 (+) | P05546 | SERPIND1 | 5 | 18.2 | 4.42 | 0.001552831 |
| 24 | Hypoxia up-regulated protein 1 (+) | Q9Y4L1 | HYOU1 | 28 | 48.4 | 1.98 | 0.001809154 |
| 25 | Inter-alpha-trypsin inhibitor heavy chain H4 (+) | Q14624 | ITIH4 | 10 | 26.5 | 3.90 | 6.04E-06 |
| 26 | Isocitrate dehydrogenase [NADP], mitochondrial (+) | P48735 | IDH2 | 20 | 60 | 2.39 | 0.001550526 |
| 27 | Lamin-B1 (+) | P20700 | LMNB1 | 20 | 54.3 | 7.74 | 2.24E-06 |
| 28 | Lamin-B2 (+) | Q03252 | LMNB2 | 10 | 49.7 | 3.77 | 1.24E-06 |
| 29 | Lipopolysaccharide-binding protein (+) | P18428 | LBP | 7 | 33.9 | 5.48 | 0.008200263 |
| 30 | Malate dehydrogenase, mitochondrial (+) | P40926 | MDH2 | 16 | 55 | 2.95 | 0.002301777 |
| 31 | NAD-dependent malic enzyme, mitochondrial (+) | P23368 | ME2 | 20 | 64 | 2.34 | 8.07E-06 |
| 32 | Neuroblast differentiation-associated protein AHNAK (+) | Q09666 | AHNAK | 23 | 32.4 | 3.08 | 6.08E-06 |
| 33 | Plastin-2 (+) | P13796 | LCP1 | 41 | 55 | 2.42 | 1.90E-04 |
| 34 | Plectin (−) | Q15149 | PLEC | 65 | 46.8 | 0.60 | 0.006498826 |
| 35 | Probable ubiquitin carboxyl-terminal hydrolase FAF-X (−) | Q93008 | USP9X | 11 | 22 | 0.51 | 7.70E-05 |
| 36 | Protein disulfide-isomerase(+) | P07237 | P4HB | 36 | 58.9 | 1.99 | 5.08E-06 |
| 37 | Ras GTPase-activating-like protein IQGAP1 (+) | P46940 | IQGAP1 | 37 | 46.7 | 2.80 | 6.60E-06 |
| 38 | Serum albumin (+) | P02768 | ALB | 42 | 64.5 | 2.91 | 0.004346685 |
| 39 | Stress-70 protein, mitochondrial (+) | P38646 | HSPA9 | 19 | 54.2 | 1.85 | 3.42E-05 |
| 40 | Vimentin (+) | P08670 | VIM | 28 | 62 | 2.07 | 0.0011167 |
| 41 | Voltage-dependent anion-selective channel protein 3 (−) | Q9Y277 | VDAC3 | 36 | 68.2 | 0.35 | 0.000430871 |
a(+), proteins increased in abundant; (−), proteins decreased in abundant. bThe differentially expressed proteins belong to mitochondrial proteins are indicated in bold. cFold change, P < 0.05 vs. the control. “*” The protein has been identified as differentially expressed protein in the blood of autistic patients in our previous study (Shen et al., 2018).