Figure 5.

Setd2 promotes PDLCs osteogenic differentiation stimulated by Sr. PDLCs were transfected by lentivirus to knockdown or over‐express Setd2, with satisfactory expressions confirmed by real‐time PCR (A) and western blot (B) analysis. Alizarin red staining (C) and quantification of mineralization (D) of PDLCs transfected by lentivirus with scrambled, Setd2‐shRNA and Setd2‐overexpressing vectors following osteogenic differentiation with or without SrCl₂. ALP staining (E) and quantification of ALP activity (F) of PDLCs transfected by lentivirus with scrambled, Setd2‐shRNA and Setd2‐overexpressing vectors following osteogenic differentiation with or without SrCl₂. (G) Expression level of osteogenic genes, Runx2, ALP, OCN of PDLCs after Setd2 knockdown or over‐expression and induction towards osteogenic differentiation with or without SrCl₂ stimulation. *P ＜ 0.5; **P ＜ 0.01; ***P ＜ 0.001