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. 2019 Mar 19;10:421. doi: 10.3389/fimmu.2019.00421

Figure 1.

Figure 1

MiR-143 and miR-365 are upregulated in Mtb HN878-infected M(IL-4/IL-13) activated mouse macrophages. (A) Timeline of mouse macrophage activation and Mtb HN878 infection. Bone marrow cells were differentiated for 10 days into BMDMs and stimulated with IFNγ or IL-4, IL-13, IL-4/IL-13. At 24 h post-stimulation, BMDMs were infected with Mtb (HN878) for 4, 24, and 48 h. RNA was extracted from lysed cells at different time points post-stimulation and post-Mtb infection. (B,C) RT-qPCR analysis of miR-143 and miR-365 kinetic expression in non-infected and Mtb-infected BMDMs. The fold change in gene expression was determined by RT-qPCR and was normalized to miR-191 expression. Non-stimulated 0 h was set to 1. Each data point represents arithmetic mean of triplicates ± SEM. Two-way ANOVA was used to evaluate statistical significance, P-values represented as, *P < 0.05, **P < 0.01, and ***P < 0.001.