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. 2019 Mar 19;10:421. doi: 10.3389/fimmu.2019.00421

Figure 2.

Figure 2

Knockdown of miR-143 and miR-365 decreases intracellular Mtb HN878 growth, CCL5 and IL-6 production and increases apoptotic death of M(IL-4/IL-13) stimulated mouse macrophages. (A) BMDMs were transfected with antagomiRs or mimics for miR-143 and miR-365, respectively. Twenty-four hours later, cells were stimulated with IL-4/IL-13 for another 24 h and subsequently infected with Mtb HN878. Cells were lysed at 4 h for uptake and 24 h post-Mtb infection to measure bacterial growth by CFU counting. (B) BMDMs were treated as described in (A), cells were harvested and stained with antibodies for active caspase-3 and Bcl2, and analyzed using FACS. (C) BMDMs were treated as described in (A). Culture supernatants were analyzed for production of CCL5 chemokine, IL-6 and TNF cytokines at 24 and 48 h post-infection using ELISA. Data represented here are mean ± SD of triplicates. A two-way ANOVA and Bonferroni post-hoc test was used to evaluate statistical significance. P-values represented as, *P < 0.05, **P < 0.01, and ***P < 0.001.