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. 2019 Mar 19;10:421. doi: 10.3389/fimmu.2019.00421

Figure 4.

Figure 4

MiR-143 targets c-Maf and Bach-1 while miR-365 targets c-Maf, Bach1, and Elmo-1 in mouse macrophages validated by mutagenesis luciferase assay. Luciferase activity of Raw264.7 cells transfected with reporter plasmid containing the 3′-UTR of c-Maf, Bach1, or Elmo-1 together with (A) miR-143 mimic, (B) miR-365 mimic or scramble control. At 72 h post-transfection, dual luciferase assay was performed to measure the ratio F-luc/R-luc. Luciferase activity of the 3′-UTR + miR NC group was set to 1. Data represent the mean ± SD of triplicate. Unpaired t-test was used to evaluate statistical significance, P-values represented as, *P < 0.05 and **P < 0.01.