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. 2019 Mar 19;10:421. doi: 10.3389/fimmu.2019.00421

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Copyright © 2019 Tamgue, Gcanga, Ozturk, Whitehead, Pillay, Jacobs, Roy, Schmeier, Davids, Medvedeva, Dheda, Suzuki, Brombacher and Guler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-143 targets c-Maf and Bach-1 while miR-365 targets c-Maf, Bach1, and Elmo-1 in macrophages validated by RNA-pulldown assay. RAW264.7 cells were transfected with biotinylated probes for (A) miR-143 mimics or (B) miR-365 mimics and whole-cell lysates were precipitated with streptavidin magnetic beads followed by RNA extraction and RT-PCR for c-Maf, Bach-1, and Elmo-1 target genes. 100 ul (20%) of the cell lysate was saved and used as input. Data are the mean ± SD of triplicate. Unpaired t-test was used to evaluate statistical significance, P-values represented as, ^*P < 0.05 and ^***P < 0.001.