Figure 6.

MiR-143 and miR-365 control the protein level of c-Maf, Bach-1, and Elmo-1 in M(IL-4/IL-13) mouse macrophages. (A) BMDMs were transfected with antagomiRs scramble, anti-MiR-143, or anti-MiR-365. At 24 h post-transfection, cells were stimulated with IL-4/IL-13 for another 48 h, then total protein was prepared from lysed cells and analyzed by western blotting, using Gapdh protein as internal control for loaded protein amount. (B) BMDMs were transfected with antagomiRs scramble, anti-miR-143 or anti-miR-365 for 24 h. Cells were then stimulated with a combination of IL-4/IL-13 for another 24 h and subsequently infected with Mtb HN878 for an additional 24 h. Total protein was prepared from lysed cells and analyzed by western blotting, using Gapdh protein as internal control.