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. 2019 Mar 25;9:5068. doi: 10.1038/s41598-019-41687-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genistein reduces expression of IL-12/18 induced expression of IL-18Rα. PBMCs from healthy human donors (n = 5) were cultured with 10 or 25 µM of genistein or DMSO (vehicle control) for 4 hours then stimulated with 20 ng/ml IL-12 and 50 ng/ml IL-18 for either 24 hours of additional incubation. IL-12 receptor (IL-12Rβ1) and IL-18 receptor (IL-18Rα) surface expression was analyzed by flow cytometry. Representative dot plots of CD56⁺ NK cells expressing (a) IL-12Rβ1 and (b) IL-18Rα. (c) IL-12Rβ1 or (d) IL-18Rα expression from 5 different donors was analyzed by fold change of unstimulated over IL-12/18 stimulated NK cells. Means +/− STD. *p < 0.01, concentration compared to DMSO control (0 μM).