Fig. 2.

CLDN19^G20D,G20D disrupted retinal neurogenesis of human induced pluripotent cells. a Schema for differentiating optic vesicles and cups. b Live cell imaging: Phase contrast (PC) images show the normal differentiation of neural rosettes and optic vesicles for CLDN19^WT,WT and CLDN19^WT,G20D, and the inability of CLDN19^G20D,G20D to form these structures. Fluorescence microscopy confirms the expression of puromycin-red fluorescent protein (RFP) in transduced clones. c Confocal images of cultures fixed in paraformaldehyde on 30 days of differentiation (DD30): CLDN19^WT,WT and CLDN19^WT,G20D expressed SIX6-GFP, an early marker of retinal differentiation, but CLDN19^G20D,G20D did not, despite the expression of puromycin-RFP. Scale bar (b) 500 µm and (c) 20 µm