Fig. 4.

The CLDN19^G20D/G20D clones form pigmented clusters inefficiently. a The schematic drawing illustrates how retinal pigment epithelium (RPE) was differentiated from neural rosettes. b Low-power and high-power images of pigmented colonies present on 80 days of differentiation (DD80). Scale bars: Top row, 1.0 cm; bottom row, 500 µm. Arrowheads indicate pigmented colony. c On DD80, pigmented colonies generated by RPE differentiation were quantified. The number of pigmented colonies formed by CLDN19^WT/G20D was similar to wild type (ns, not statistically significant). CLDN19^G20D/G20D produced significantly fewer. Mean values represent number of pigmented colonies in each dish (4 dishes). Error bars ± SEM; ***P < 0.001