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. 2019 Mar 25;9:5048. doi: 10.1038/s41598-019-41556-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) C30-RPLC-HESI-MS chromatogram of rat brain lipids analyzed in positive ion mode. (b) Extracted ion chromatogram (XIC) showing separation of TG 54:6 isomers in rat brain, (c–e) C30-RPLC-HRMS/MS spectra of m/z 896.77 [M + NH₄]⁺ precursor ion representing three isomers of (c) TG 18:2/18:2/18:2, (d) TG 16:0/20:4/18:2 and (d)TG 16:0/22:6/16:0 eluting at 25.10, 25.60 and 26.21 minutes, respectively.