Table 2.
Estimates of LT50 and LD50 of the Pleurotus aegerolysin/PlyB protein complexes against the WCR larvae and beetles and the CPB larvae.
| Sample | Treatment | LT50 (days)a | Hazard ratiob | χ2,c | P-valuec | LD50 (µg/cm2)d | ||
|---|---|---|---|---|---|---|---|---|
| Mean ± SE | 95% CI | Mean ± SE | 95% CI | |||||
| WCR larvae | OlyA6/PlyB | 4.1 ± 0.4 | 3.4–4.9 | 8.4 | 60.4 | <0.0001 | 7.8 ± 3.6 | 0.21–15.4 |
| PlyA2/PlyB | 2.3 ± 0.3 | 1.7–2.8 | 37.2 | 84.1 | <0.0001 | 7.4 ± 3.4 | 0.24–14.5 | |
| EryA/PlyB | 7.4 ± 0.3 | 6.7–8.1 | 2.7 | 4.1 | >0.05 | |||
| 0.5% Decise | 0.6 ± 0.001 | ND | 3670 | 70.8 | <0.0001 | |||
| Buffer | 12 ± 2.9 d | 5.7–18 | ||||||
| WCR beetles | OlyA6/PlyB | 5.0 ± 0.58 | 3.7–6.2 | 5.0 | 22.0 | <0.0001 | ||
| PlyA2/PlyB | 8.3 ± 1.6 | 4.9–12 | 1.2 | 0.1 | >0.05 | |||
| EryA/PlyB | 8.9 ± 1.9 | 4.7 13 | 1.3 | 0.4 | >0.05 | |||
| 0.5% Decise | ~0.38 | ND | 70.5 | 65.0 | <0.0001 | |||
| Buffer | 8.4 ± 1.1 | 6.0–11 | ||||||
| CPB larvae L1 + L2 | OlyA6/PlyB | 4.3 ± 0.5 | 3.3–5.4 | 8.5 | 22.4 | <0.0001 | ||
| PlyA2/PlyB | 4.6 ± 0.6 | 3.3–6.0 | 6.7 | 15.9 | <0.0001 | |||
| EryA/PlyB | 4.9 ± 0.4 | 3.9–5.9 | 7.3 | 14.0 | 0.0002 | |||
| 0.1% Actaraf | ~0.8 | ND | 111 | 98.3 | <0.0001 | |||
| Buffer | 6.7 ± 1.1 | 4.5–9.0 | ||||||
| CPB larvae L3 + L4 | OlyA6/PlyB | 4.5 ± 0.3 | 3.9–5.1 | 8.1 | 20.8 | <0.0001 | ||
| PlyA2/PlyB | 4.1 ± 0.3 | 3.5–4.7 | 12.3 | 32.6 | <0.0001 | |||
| EryA/PlyB | 5.4 ± 0.4 | 4.6–6.2 | 4.0 | 6.1 | >0.05 | |||
| 0.1% Actaraf | ~0.9 | ND | 129 | 106 | <0.0001 | |||
| Buffer | 9.6 ± 1.8 | 2.9–31.6 | ||||||
aWCR larvae were exposed to 5.6 µg/cm2 aegerolysin/PlyB. WCR beetles were exposed to 0.5 mg/mL aegerolysin and 0.04 mg/mL PlyB. CPB larvae were exposed to 9.0 µg/cm2 aegerolysin/PlyB. bHazard ratio is defined as the slope of the survival curve, as a measure of how rapidly the subjects died. The hazard ratio was computed for each treatment from the survival curves, compared to the buffer (negative control). cχ2 (log-rank test) was computed separately for each pairwise comparison of treatment versus buffer (negative control), and consequently its corresponding P-value compared to Bonferroni-corrected significance thresholds (P/K, where P = 0.05 and K is the number of pairwise comparisons). If the P-value exceeded the Bonferroni-corrected significance threshold, it is reported as >0.05. dLD50 of OlyA6/PlyB and PlyA2/PlyB assessed only for WCR larvae at day 7. eInsecticide based on active ingredient: deltamethrin. fInsecticide based on active ingredient: thiametoxam. ND, not determined.