Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 25;9:5037. doi: 10.1038/s41598-019-41661-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Development of spontaneous synaptic transmission follows lamina-specific patterns. (a) Example traces recorded at −70 mV (left) or +10 mV (right) from L5PNs at 1, 2 and 4 weeks. Bottom panels show probability distributions for inter-event-intervals and charge-per-event for single example cells. (b) sEPSC frequency increases in L3 during the second postnatal week (Welch’s F(2,17.8) = 36.1, p < 0.001; post-hoc w1–2, p < 0.001). sEPSCs frequency in L5 increases between weeks 1 and 4 (Welch’s F(2,13.3) = 30.13, p < 0.001; post-hoc w1–2, p = 0.006; w2–4, p = 0.001). (c) sEPSC charge/second increases from week 1 to 4 in L3 (Welch’s F(2,16.3) = 15.70, p = 0.002; w1-w4, p = 0.001) and from week 2 to 4 in L5 (Welch’s F(2,16.0) = 15.49, p < 0.001; w2–4, p < 0.001). (d) sIPSC frequency increases in L3 between weeks 2 and 4 (W’s F(2,17.0) = 23.53, p < 0.001; w2–4, p < 0.001) and until week 2 in L5 (F(2,34) = 15.58, p < 0.001; w1–2, p = 0.029; w2–4, p = 0.005). L3-L5 w2, t(31) = 3.19, p = 0.003. (e) sISPCs charge/second increases in week 2 to 4 in L3 (F(2,35) = 23.80, p < 0.001; w2–4, p < 0.001), and from week 1 to 4 in L5 (W’s F(2,17.9) = 18.22, p = 0.018; w1–2, p = 0.029; w1–4, p = 0.005). L3-L5 w2, M-W U = 76, p = 0.031. (f) Ratio of sEPSC and sIPSC frequencies measured in the same cells. In L3, E/I frequency decreases between weeks 2 and 4 (K-W H(3) = 13.57, p = 0.001; w2–4, p < 0.001). In L5, E/I frequency ratio increases between weeks 1 and 4 (F(2,34) = 1.60, p = 0.033; w1–4, p = 0.047). L3 vs L5: w2, Welch’s t(19.05) = 5.167, p < 0.001; w4, t(18) = 0.07, p = 0.948. (g) Ratio of sEPSC/sIPSC charge in L3 decreases between weeks 2 and 4 (K-W H(3) = 9.92, p = 0.007; w2–4, p = 0.005). In L5 cells, there is an increase between weeks 1 and 4 (F(2,34) = 1.64, p = 0.011; w1–4, p = 0.008). L3 vs L5: w2, Welch’s t(24.84) = 3.005, p = 0.006; w4, M-W U = 21, p = 0.029.