Table 2.
Electrophysiological properties of layer 3 and 5 pyramidal neurons at 1, 2, and 4 weeks. Ages are given as mean ± sd in days postnatal.
| Variable | Week 1 p 7.0 ± 0.9 n = 10/5 | Week 2 p 14.0 ± 0.0 n = 15/5 | Week 4 p 28.6 ± 1.0 n = 16/7 | Omnibus test result |
|---|---|---|---|---|
| Layer 3 | ||||
| Resting membrane potential (mV) | −61.63 ± 1.39*** | −69.96 ± 0.98 | −73.02 ± 1.09 | F(2,38) = 23.42 p < 0.001 |
| Input resistance (MΩ) | 405.85 ± 50.54*** | 213.42 ± 21.95*** | 110.28 ± 11.54 | F(2,10) = 14.09 p = 0.001 |
| Membrane time constant (ms) | 0.47 ± 4.32*** | 40.23 ± 2.48*** | 19.87 ± 2.34 | F(2,36) = 45.42 p < 0.001 |
| Action potential threshold (mV) | −32.69 ± 0.75*** | −37.68 ± 0.44 | −39.11 ± 0.54 | F(2,38) = 31.36 p < 0.001 |
| Action potential amplitude (mV) | 64.76 ± 2.26*** | 85.41 ± 1.37 | 88.91 ± 1.78 | F(2,37) = 46.85 p < 0.001 |
| Halfwidth (ms) | 2.05 ± 0.14** | 1.27 ± 0.07* | 1.01 ± 0.05 | W’s F(2,18) = 25.61 p < 0.001 |
| Rheobase (pA) | 27.56 ± 5.55* | 57.10 ± 6.17*** | 105.47 ± 10.32 | F(2,35) = 23.13 p < 0.001 |
| ISI 1/ISI 9 | 0.58 ± 0.09* | 0.41 ± 0.05 | 0.30 ± 0.05 | F(2,31) = 5.21 p = 0.011 |
| ISI 4/ISI 9 | 0.84 ± 0.05 | 0.90 ± 0.02 | 0.90 ± 0.02 | F(2,30) = 1.42 p = 0.258 |
| Sag (%) | 8.08 ± 1.78 | 5.27 ± 0.91 | 5.05 ± 0.59 | W’s F(2,18) = 1.26 p = 0.307 |
| Layer 5 | ||||
| Variable | Week 1 p 7.1 ± 0.9 n = 12/5 | Week 2 p 14.0 ± 0.0 n = 19/6 | Week 4 p 28.7 ± 1.0 n = 15/8 | Omnibus test result |
| Resting membrane potential (mV) | −61.30 ± 1.45*** | −67.08 ± 0.63 | −69.62 ± 0.83 | F(2,43) = 18.39 p < 0.001 |
| Input resistance (MΩ) | 161.83 ± 10.06** | 78.33 ± 3.68* | 52.60 ± 5.73 | H(2) = 29.33 p < 0.001 |
| Membrane time constant (ms) | 45.33 ± 2.88*** | 19.01 ± 0.99*** | 11.73 ± 0.80 | F(2,12) = 50.11 p < 0.001 |
| Action potential threshold (mV) | −35.32 ± 0.56*** | −41.89 ± 0.64 | −43.57 ± 0.71 | F(2,43) = 36.87 p < 0.001 |
| Action potential amplitude (mV) | 75.24 ± 1.62*** | 91.54 ± 1.17 | 94.22 ± 1.61 | F(2,43) = 43.78 p < 0.001 |
| Halfwidth (ms) | 2.14 ± 0.08*** | 1.18 ± 0.04** | 0.95 ± 0.03, n = 15) | F(2,42) = 124.67 p < 0.001 |
| Rheobase (pA) | 48.27 ± 10.01*** | 128.15 ± 7.57** | 184.47 ± 17.48 | F(2,40) = 29.74 p < 0.001 |
| ISI 1/ISI 9 | 0.50 ± 0.07 | 0.50 ± 0.05 | 0.51 ± 0.07 | F(2,37) = 0.001 p = 0.999 |
| ISI 4/ISI 9 | 0.79 ± 0.04*** | 1.03 ± 0.04 | 1.06 ± 0.03 | F(2,38) = 23.86 p < 0.001 |
| Sag (%) | 31.52 ± 3.20** | 18.76 ± 1.23 | 20.13 ± 1.72 | W’s F(2,22) = 6.72 p = 0.005 |
N given as number of cells/number of mice. Values are given as mean ± SEM. W’s F = Welch’s F. Asterisks denote post-hoc significance: *p < 0.05, **p < 0.01, ***p < 0.001. Bold values denote statistically significant differences compared to week 2 (in week 1), italics denote statistically significant differences compared to week 4 (in week 1 and 2).