Table 3.
Properties of excitatory and inhibitory synaptic input onto layer 3 and 5 pyramidal neurons at 1, 2, and 4 weeks.
| Variable | 1 week p 7.2 ± 0.8 n = 11/8 | 2 weeks p 14.6 ± 1.0 n = 12/7 | 4 weeks p 28.2 ± 0.9 n = 10/7 | Omnibus test result |
|---|---|---|---|---|
| Layer 3 | ||||
| sEPSC frequency (Hz) | 1.39 ± 0.29*** | 6.36 ± 0.84 (n = 17/10) | 8.81 ± 1.00 | W’s F(2,17.8) = 36.1 p < 0.001 |
| sEPSC charge (pC/s) | 170.8 ± 35.8** | 732.1 ± 187.7 (n = 17/10) | 1209.6 ± 204.7 | W’s F(2,16.3) = 15.70 p = 0.002 |
| sEPSC amplitude (pA) | 32.05 ± 2.28* | 23.53 ± 0.96 | 26.58 ± 1.37 | W’s F(2,18) = 6.26 p = 0.009 |
| sEPSC rise time (ms) | 0.56 ± 0.02** | 0.73 ± 0.05 | 0.79 ± 0.05 | F(2,10) = 9.17 p = 0.005 |
| sEPSC decay time (ms) | 2.97 ± 0.21* | 3.15 ± 0.13 | 3.71 ± 0.15 | F(2,30) = 5.22 p = 0.011 |
| sIPSC frequency (Hz) | 0.66 ± 0.19*** | 2.65 ± 0.46*** | 9.59 ± 1.50 | W’s F(2,17.0) = 23.53 p < 0.001 |
| sIPSC charge (pC/s) | 574.2 ± 208.3** | 1159.1 ± 316.6 ** | 6098.4 ± 1,163.9 | K-W H(3) = 22.27 p < 0.001 |
| sIPSC amplitude (pA) | 42.38 ± 2.96* | 40.13 ± 2.54** | 56.71 ± 5.05 | H(2) = 8.30 p = 0.016 |
| sIPSC rise time (ms) | 1.20 ± 0.11 | 1.14 ± 0.10 | 1.48 ± 0.15 | F(2,30) = 2.21 p = 0.128 |
| sIPSC decay time (ms) | 17.71 ± 0.70 *** | 13.57 ± 0.51** | 10.68 ± 0.36 | W’s F(2,19) = 41.03 p < 0.001 |
| Layer 5 | ||||
| Variable | 1 week p 7.0 ± 0.8 n = 11/9 | 2 weeks p 14.7 ± 0.9 n = 11/7 | 4 weeks p 28.1 ± 1.1 n = 10/8 | Omnibus test result |
| sEPSC frequency (Hz) | 1.28 ± 0.20** | 4.75 ± 0.06** (n = 16/10) | 9.22 ± 1.29 | W’s F(2,13.3) = 30.13 p < 0.001 |
| sEPSC charge (pC/s) | 213.6 ± 33.7*** | 596.2 ± 115.9*** (n = 16/10) | 1683.8 ± 303.9 | W’s F(2,16.0) = 15.49 p < 0.001 |
| sEPSC amplitude (pA) | 29.36 ± 1.82** | 24.44 ± 1.37 | 34.49 ± 2.19 | F(2,27) = 7.59 p = 0.002 |
| sEPSC rise time (ms) | 0.74 ± 0.08 | 0.84 ± 0.05 | 0.88 ± 0.07 | H(2) = 3.57 p = 0.167 |
| sEPSC decay time (ms) | 3.88 ± 0.44 | 3.70 ± 0.24 | 3.61 ± 0.11 | W’s F(2,15) = 0.22 p = 0.807 |
| sIPSC frequency (Hz) | 2.10 ± 0.34* | 5.14 ± 0.64** | 9.12 ± 1.38 | W’s F(2,17.9) = 18.22 p < 0.001 |
| sIPSC charge (pC/s) | 1859.4 ± 409.1 * | 2389.5 ± 371.1 | 3921.7 ± 650.1 | F(2,33) = 0.46 p = 0.018 |
| sIPSC amplitude (pA) | 46.10 ± 2.75 | 40.13 ± 1.34 | 47.27 ± 2.58 | F(2,27) = 2.63 p = 0.090 |
| sIPSC rise time (ms) | 1.29 ± 0.15* | 1.08 ± 0.05* | 0.85 ± 0.05 | W’s F(2,17) = 6.85 p = 0.007 |
| sIPSC decay time (ms) | 16.72 ± 1.40** | 10.21 ± 0.60 | 8.75 ± 0.71 | W’s F(2,18) = 12.42 p < 0.001 |
Ages are given as mean ± sd in days postnatal. N given as number of cells/number of mice. Values are given as mean ± SEM. W’s F = Welch’s F. Asterisks denote post-hoc significance: *p < 0.05, **p < 0.01, ***p < 0.001. Bold values denote statistically significant differences compared to week 2 (in week 1), italics denote statistically significant differences compared to week 4 (in week 1 and 2).