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. 2019 Mar 25;10(4):281. doi: 10.1038/s41419-019-1515-3

Fig. 4. CADM1 decreases cellular motility and invasiveness.

Fig. 4

a Western blot analysis was used to validate CADM1 expression levels of doxycycline inducible 1205LuTR cells engineered to express control LacZ, CADM1, or CADM1 shRNAs. b Cells from (a) were subjected to matrigel-coated Boyden chamber invasion assays. Quantification of invaded cells, and representative images are shown. Data is graphed as a fold change compared to 1205LuTR LacZ invaded cells from at least three independent experiments, * represents p < 0.05 as determined by t-test, and error bars are ±SEM. Scale bar = 200 μM (c) Similar to (b), 1205LuTR cells were subjected to Boyden chamber based, serum directed migration assays. Scale bar = 200 μM (d, e) Spheroids of LacZ or CADM1 expressing WM793TR (d) and 1205LuTR (e) cells were assayed for their invasiveness into collagen. Representative images of phase contrast and green fluorescence via Calcien AM staining are shown. Invasive index is quantified from at least N = 40 per group across three different experiments. * represents p < 0.05 as determined by t-test, error bars are ±SEM. Scale bar = 200 μM. f Similar to (d) except that melanoma cell invasiveness was assayed in 3D skin reconstruct model. The depth of melanoma was measured from at least 70 measurements across two experiments. * represents p < 0.05 as determined by t-test, error bars are ±SEM. Scale bar = 200 μM