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. 2019 Mar 25;10(4):281. doi: 10.1038/s41419-019-1515-3

Fig. 5. CADM1 induces cell death in non-adherent conditions.

Fig. 5

a WM793TR, 1205LuTR, and A375TR cells expressing LacZ or CADM1 were measured for cell death via annexin V/propidium iodine positivity. After overnight seeding, cell lines were treated with doxycycline to induce transgene expression for 24 h. Cells were harvested and assayed for annexin V/propidium iodine via flow cytometry. Data are represented as fold alive (unstained) compared to LacZ expression as a control from at least three independent experiments, * represents p < 0.05 as determined by t-test, and error bars are ±SEM. b Similar to (a) except that cells were seeded in bacto-agar coated wells. c WM793TR LacZ and CADM1 cells were seeded in bacto-agar coated wells with or without an injection of 80–100 μg of Matrigel. Data are represented as fold alive (unstained) compared to WM793 LacZ without Matrigel as a control from at least three independent experiments, * represents p < 0.05 as determined by t-test, and error bars are ±SEM. d Representative images after 21 days of colony growth for WM793TR LacZ or CADM1 cells in soft agar. Number of colonies per field and colony size are quantified. * represents p < 0.05 as determined by t-test, error bars are ±SEM. Scale bar = 200 μM