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Comparison of ZFN, TALEN, and CRISPR.
| Property | ZFN | TALEN | CRISPR |
|---|---|---|---|
| Anchor site | 18–36 nt | 30–36 nt | 23 nt |
| Off-target | Low | low | High |
| Complication | High | High | Low |
| Efficiency | Relatively low | Relatively low | High |
| Multiplex | Low | Low | High |
| Methylation sensitivity | High | High | Low |
| Mechanism of action | Zinc finger nuclease for DNA recognition and cleavage | transcription activator-like effector nuclease recognition and DNA cleavage | Guide RNA for DNA recognition and Cas9 endonuclease for cleavage |
