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. 2019 Mar 25;10(4):284. doi: 10.1038/s41419-019-1504-6

Fig. 2. BAG3 overexpression induces autophagy independent of Beclin 1 and PtdIns3K.

Fig. 2

a Control or BAG3-overexpressing HepG2 and MCF7 cells were transfected with scramble shRNA or shRNA specific against Beclin1 (shBeclin1), and Western blot analysis was performed using the indicated antibodies. b HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct were treated with vehicle, 3-MA or WM respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Then cells were treated with vehicle, 3-MA or WM, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of (c). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. *P < 0.05. N.S. not significant. Error bars indicate means ± SD