Skip to main content
. 2019 Mar 19;13:143. doi: 10.3389/fnins.2019.00143

FIGURE 2.

FIGURE 2

Morphology, actin organization and conditioned media of C6/C6-13. Subconfluent cultures of C6 (A) and C6-13 (B) cells. C6 present a spindle appearance, while C6-13 are flat and exhibit membrane ruffles and protrusions (arrows). (C,D) Actin (red) organization under scrape-wound conditions (-FBS, 15 h). (C) C6 actin stress fibres presented a stellate, random orientation at the wound gap. (D) C6-13 actin fibres were aligned between cells and parallel with the movement into the space. Nuclei were co-stained by DAPI (blue) and the cell proliferation marker Ki-67 (green). Bar: 20 μm. (E) C6-13 conditioned medium exhibited a higher viscosity that was readily observed during aspirations. (F) Conditioned media viscosity measurements. Box and whisker plot of timed laminar flow demonstrate increased viscosity of C6-13 conditioned media. Whiskers designate absolute max/min values (N = 8); significance using Student’s T (∗∗p ≤ 0.01).