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. 2019 Mar 19;13:143. doi: 10.3389/fnins.2019.00143

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Copyright © 2019 Aftab, Mesnil, Ojefua, Poole, Noordenbos, Strale, Sitko, Le, Stoynov, Foster, Sin, Naus and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Secretome analysis identifies differentially expressed proteins. (A) Volcano plot of secretome proteins (T-test P-value, and log₂ ratio) to identify differential expression. These proteins include reelin, MMP3 and osteopontin. Select MS/MS and MS spectra: (B) Reelin; (C) MMP3; and (D) Osteopontin showing identity and differential increases with C6/C6-13. (E) SDS-PAGE/Western blot of lysates and conditioned media (CM) supports increases in osteopontin in C6-13. (F) Table summarizing proteins found to be differentially expressed: fold-change ratio, P-value, anticipated cellular location, function/enzyme type and presence of a protein secretion signal.