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. 2019 Mar 19;13:143. doi: 10.3389/fnins.2019.00143

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Copyright © 2019 Aftab, Mesnil, Ojefua, Poole, Noordenbos, Strale, Sitko, Le, Stoynov, Foster, Sin, Naus and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binary increases of osteopontin and MMP3 in high motility C6-13. Targeted detection of (A) osteopontin/SPP1 and (D) MMP3 monitored by HR-MRM in biological triplicates of C6/C6-13 conditioned media. (B,E) Sequence, parent and fragment ions (m/z) used to monitor MMP3/osteopontin proteins. Significant increases of (C) osteopontin and (F) MMP3 are observed with C6-13 secretome (^∗p ≤ 0.05, ^∗∗∗p ≤ 0.001). (G,H) Fibronectin observed in C6/C6-13 was used to monitor sample load and signal normalization.