Figure 3.

Morphological analysis of recombinant L. donovani populations. L. donovani promastigotes were seeded at 1 × 10⁶ cells ml-1 in Medium199 containing radicicol at IC₉₀ (batch-dependent; 0,5-1,25 μg/ml) and allowed to proliferate for 72 h. The cells were fixed and then visualised by SEM or fluorescence microscopy using anti-α-tubulin mAb. By using image analysis, the cell body lengths of 50 (in µm, upper panels), the cell body widths of 25 (in µm, middle panels) and the flagella lengths (in µm, lower panels) of 25 randomly selected cells were analysed and mean values were compiled. Bars represent the mean measurements with SEM, n = 25. (n.d.): not detectable, (n.s.): not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, respectively, by U-test. (A) analysis performed by using SEM images. (B) analysis performed by using fluorescence microscopy images. (C) SEM images of HSP90rr and HSP90rr-T100D expressing cells -RAD and + RAD.