Table 2.
Compilation of results. Relative (in % of HSP90rr values) in vitro growth, morphology, infectivity and substrate potential for CK1.2 of the tested mutants.
| domains | AA | to | growth [%] | length [%] | width [%] | flagellum [%] | infectivity[%] | CK1.2 Substrate |
|---|---|---|---|---|---|---|---|---|
| HSP90rr | 100.0 | 100 | 100 | 100 | 100 | n.d. | ||
| HSP90 | 7.4 | 71 | 189 | 78 | 25 | + | ||
| N domain | Thr 21 | I | 7.3 | 70 | 229 | 75 | 29 | ± |
| D | 5.1 | 59 | 247 | 61 | 11 | n.d. | ||
| Thr 100 | I | 6.3 | 69 | 221 | 53 | 38 | ± | |
| D | 3.9 | n.d. | n.d | nd | 10 | n.d. | ||
| CL domain | Thr 211 | A | 72.9 | n.d. | n.d | nd | 79 | n.d. |
| D | 69.2 | n.d. | n.d | nd | 54 | n.d. | ||
| Thr 216 | A | 69.6 | n.d. | n.d | nd | 61 | n.d. | |
| D | 69.4 | n.d. | n.d | nd | 114 | n.d. | ||
| Thr 211, 216 | A | 52.9 | 76 | 120 | 64 | − | ||
| Thr 211, 216 | D | 37.4 | n.d. | n.d. | n.d. | n.d. | n.d. | |
| Thr 223 | A | 76.0 | 74 | 111 | 85 | 57 | − | |
| D | 62.0 | 94 | 134 | 51 | 61 | n.d. | ||
| M domain | Ser 289 | A | 47.5 | 75 | 123 | 54 | 66 | + |
| Ser 526 | A | 71.6 | 80 | 100 | 54 | 76 | − | |
| C domain | Ser 594 | A | 58.1 | 91 | 111 | 80 | 65 | n.d. |
| D | 6.3 | 76 | 156 | 57 | 3 | n.d. | ||
| Ser 595 | A | 74.0 | 98 | 117 | 87 | 70 | n.d. | |
| D | 98.6 | 95 | 125 | 79 | 80 | n.d. | ||
| Ser 594/5 | A | n.d. | n.d. | n.d. | n.d. | − | ||
| D | n.d. | n.d. | n.d. | n.d. | n.d. | |||
| Thr 693 | A | 77.9 | 94 | 125 | 80 | 91 | ± | |
| D | 93.1 | 94 | 132 | 92 | 81 | n.d. | ||
| Ser 694 | A | 88.2 | 97 | 112 | 85 | 93 | ± | |
| D | 6.5 | 65 | 217 | 60 | 4 | n.d. |