Fig. 3. Cas9 nickase shows lower off-target activity with loss of target editing efficiency.

Schematic of RAG1A, RAG1B, RAG1C, and RAG1G target site positions. Purple arrows indicated the biotinylated primers used for primer-extension-mediated sequencing. Yellow boxes represented guide RNA target sites and red bars indicated Cas9 cleavage site. In the lower panels, pie graph showed the compositions of germline, indels, and translocation for indicated Cas9 treatment in HEK293T cells. Mean ± SD