Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 25;9:5112. doi: 10.1038/s41598-019-41382-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of ADR expression in different Arabidopsis organs and GUS staining patterns in ADR::GUS Arabidopsis flowers. (A) The detection of ADR expression in different Arabidopsis organs. The mRNA levels were determined by RT-PCR. Total RNA was isolated from 1-week-old seedlings (1W), 2-week-old seedlings (2W), rosette leaves (RL), cauline leaves (CL), roots (Rt), stems (St), floral buds (FB) and siliques (Si). The Arabidopsis ACTIN8 (ACT8) gene was used as internal controls. The grouping of two gels (ADR and ACT8) was cropped from two different original gels in Fig. S4. (B) The detection of ADR expression in wild-type flowers at two different developmental stages (8–11, >12). The mRNA levels were determined by real-time quantitative PCR. (C) In stage 11 of ADR::GUS young floral buds, GUS activity was strongly detected in the sepals (s) and anthers (an) of stamens but relatively weakly detected in the petals (p), carpels (c) and filaments (f) of stamens. (D) Close-up of the anther (an) from (C). (E) In stage 13 of ADR::GUS mature flowers, GUS was strongly detected in sepals (s), petals (p) and carpels (c). In the stamen, GUS activity was detected in the filaments (f) but was absent in the anthers (an) of stamens. (F) Close-up of the anther (an) from (E).