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. 2019 Mar 25;9:5112. doi: 10.1038/s41598-019-41382-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient expression of ADR + GFP and CAT3 + mORG2 in tobacco cells. (A–D) Agrobacterium-mediated transient expression of ADR + GFP (A,B) and CAT3 + mORG2 (C,D) in epidermal cells of N. benthamiana. ADR + GFP fusion protein accumulated in organelle-like structures (arrowed in A,B) that were highly similar to peroxisomes, where CAT3 + mORG2 were localized (arrowed in C,D). (E,F) A merged fluorescence image of (A and C) in (E); (B and D) in (F) showed the co-localization of ADR + GFP and CAT3 + mORG2 (green-yellow color) in peroxisomes (arrowed). (B,D and F) are the close-up images from (A,C and E), respectively. Scale bars: 30 μm in (A,C and E) and 5 μm in (B,D and F).