Figure 3.

Akt activates Nrf2 in EBV-transformed B cells. (A) Cell extracts were prepared from PBMCs and EBV-transformed B cells. The protein levels of pAkt and Akt were measured by Western blot analysis. (B-D) EBV-transformed B cells were treated with LY294002 (10 μM) for 48 hours. (B and D) Whole cell extract and (C) cytosolic and nuclear extracts were prepared from LY294002-treated cells. The protein levels of Nrf2, pAkt, Akt, HO-1, and NQO-1 were measured by Western blot analysis. β-Actin and Lamin B1 were used as equal loading controls for normalization. CE, cytosol extract; NE, nuclear extract. (E) Cell extracts were prepared from siRNA-LMP1 or LMP2A-transfected EBV-transformed B cells. The protein levels of pAkt and Akt were measured by Western blot analysis. The fold increase in pAkt, Nrf2, NQO-1, and HO-1 is indicated numerically, as determined by densitometry.