Supplementary Figure 2.

Nrf2 translocation into nucleus is effected by the expression of LMP1 with mutation CTAR1 regions. Cytosolic and nuclear extracts were prepared from 293T cells were transfected with LMP1-△194-232 or wild type (WT) vector plasmid. 48 h later, the expression of the WT and mutant LMP1 proteins were determined by Western blot analysis. b-actin and Lamin B1 were used as equal loading control for normalization. CE: cytosol extract, NE: nuclear extract.