Supplementary Figure 4.

Inhibition of Nrf2 activation by LMP1 or LMP2A or LY294002 was not affect from ubiquitination-mediated degradation. (A) Whole cell extracts were prepared from PBMCs, EBVtB cell and MG132 treated EBVtB cells. EBV-transformed B cells were transfected with siRNA against (B) LMP1 or (C) LMP2A or (D) treated LY294002 and/or with MG132 (5 mM) for 48 h. The protein levels of Nrf2 were measured by Western blot analysis. b-actin were used as equal loading controls for normalization.