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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CFZ on cell morphology and viability of SK-N-BE(2)-M17 cells. (A) SK-N-BE(2)-M17, IMR-32, SH-SY5Y, SK-N-SH, SK-N-MC, and Neuro-2A (N2A) cells were treated with vehicle or various concentrations of CFZ for 24 h. Cell viability was assessed by the MTT assay. The percentages of cell viability are plotted as the mean ± standard deviation of at least three experiments. All data points are statistically (P < 0.05) significant compared to the vehicle-treated control (not shown). (B) Representative photomicrographs showing morphological changes in SK-N-BE(2)-M17 cells treated with vehicle (DMSO) or various concentrations (100–400 nM) of CFZ for 24 h.