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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of CFZ on MMP loss and ROS production in SK-N-BE(2)-M17 cells. SK-N-BE(2)-M17 cells were treated with vehicle or 200 nM CFZ for 1, 6, 12 and 24 h. The cells were then stained with either 10 μM DiOC₆ for 15 min (A) or 25 μM DCFH-DA for 30 min (B) and the fluorescence intensity was measured by flow cytometry. Data on the marked region indicate the percentage of cells with MMP loss (A) and ROS production (B).