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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CFZ on autophagy markers in SK-N-BE(2)-M17 cells. (A) SK-N-BE(2)-M17 cells were treated with 200 nM CFZ for the indicated times. Total cell extracts were analyzed by SDS-PAGE and western blot with antibodies against autophagy marker proteins, such as LC3BI/II, p62-SQSTM, and β-actin. (B–D) The band density was analyzed by densitometry of each band and plotted as the relative level (%) of LC3BII (B) and p62-SQSTM (D), the ratio of LC3BII/LC3BI (C). **P < 0.01 and ***P < 0.001, compared to the vehicle-treated control.