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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

Figure 6.

Figure 6

Effects of NAC on CFZ-induced ROS production, cell viability, and death-associated proteins in SK-N-BE(2)-M17 cells. (A) To measure ROS production, cells were pretreated with vehicle or 5 mM NAC for 1 h before treatment with 200 nM CFZ in SK-N-BE(2)-M17 cells. After 6 and 24 h treatment with vehicle or CFZ in the presence of NAC, cells were stained with 25 μM DCFH-DA for 30 min. The stained cells were analyzed by flow cytometry. (B) The NAC-pretreated cells were treated with 200 nM CFZ and the cell extracts were analyzed by western blotting with antibodies against cleaved caspase-3, GRP94, XBP1, LC3BI/II, and β-actin. (C) Cells were pretreated with vehicle (black bar) or 5 mM NAC (gray bar) for 1 h before treatment with CFZ (200 nM) in SK-N-BE(2)-M17 cells. Cell viability was measured by the MTT assay. The percentage cell viabilities were plotted.