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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

Figure 7.

Figure 7

Additive effects of Cis and CFZ on cell viability, cell cycle arrest, and apoptosis in SK-N-BE(2)-M17 cells. (A) SK-N-BE(2)-M17 cells were treated with various concentrations (2–20 μM) of Cis in the absence (vehicle) or presence of 50 nM CFZ for 24 h. Cell viability was measured by the MTT assay. The percent cell viabilities are plotted. *P < 0.05, **P < 0.01, and ***P < 0.001, compared to the vehicle-treated control. (B) To examine cell cycle arrest, cells were treated with vehicle, 100 nM CFZ, 10 μM Cis, or 100 nM CFZ +10 μM Cis for 24 h. (C) Cells were treated with 50–100 nM CFZ, 10–20 μM Cis, or various combinations of CFZ + Cis. The cells were then stained with PI and annexin V-FITC, and apoptotic cells were evaluated by flow cytometry. (D) CFZ alone- or Cis alone-treated or co-treated cells were lysed and the cell extracts were analyzed by western blotting with antibodies against cleaved caspase-3, GRP78, CHOP, and β-actin.