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. 2019 Mar 25;9:5039. doi: 10.1038/s41598-019-41527-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in cell viability by CFZ treatment in Cis-resistant SK-N-BE(2)-M17 cells. Cis-resistant SK-N-BE(2)-M17 cells were prepared by treatment with 2 μM Cis for 20 days or longer in the 10% FBS culture media. The culture medium was refreshed after every 2 days. Control non-resistant cells and Cis-resistant cells were treated with various concentrations of Cis alone (1–10 μM), CFZ alone (5–50 nM), or various combinations of CFZ + Cis for 48 h. Cell viability was analyzed by the MTT assay and the percentage cell viabilities were plotted. **P < 0.01, compared to the vehicle-treated control.