FIG 6.

Representative histological architecture of the colons of mice that received i.p. injection of 50 μg of A. caninum LMWM-ESP. Longitudinal sections of mouse colon were stained with hematoxylin & eosin. (A) Naive or untreated control colon displayed healthy crypts and goblet cells (green arrow) and lamina propria (black arrow); for TNBS-only treated colon, note the destruction of the mucosal layer, disappearance of goblet cells (green arrow), and thickening of lamina propria (black arrow); for the colon of a mouse treated with LMWM-ESP prior to administration of TNBS, note the maintenance of mucosal layers and relatively little immune infiltration of the tissue. (B) Aggregate blinded scoring of histopathology sections, where a score of 0 is completely normal tissue and 16 means complete destruction of tissue architecture. Statistical analyses were performed using GraphPad Prism 7 (one-way ANOVA).