FIG 3.

Effects of mepacrine on CH-1 complex formation in the intestine. Mouse small intestinal loops received an injection of 1 ml of HBSS containing 100 μg of CPE plus one of two concentrations of mepacrine (0.5 or 1.0 mM), 100 μg of CPE alone, or 1.0 mM mepacrine alone for 4 h. Intestinal tissue was then homogenized by sonication in RIPA buffer containing protease inhibitor cocktail plus 1 μl Benzonase (Qiagen). The homogenates were then analyzed by SDS-PAGE and Western blotting with a rabbit anti-CPE antibody.