Deletion of lpxO decreases A. baumannii resistance to CAMPs. Shown is percent survival of A. baumannii ATCC 17978, A. baumannii ΔlpxO mutant (ΔlpxO), and A. baumannii ΔlpxO::Tn7lpxO mutant (ΔlpxO::Tn7lpxO) following 1 h of exposure to polymyxin B (A), colistin (B), and human β-defensin 3 (C). Values are presented as the means ± standard deviations (SD) from three independent experiments measured in duplicate. P values were <0.01 (***) and <0.001 (****) for the indicated comparisons using one-way ANOVA with Bonferroni contrasts; n.s., not significant.