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. 2019 Mar 25;87(4):e00066-19. doi: 10.1128/IAI.00066-19

FIG 7.

FIG 7

lpxO deletion in A. baumannii results in a decrease in il10 expression. (A) il10 expression in iBMDMs infected for 5 h with UV-killed A. baumannii ATCC 17978, A. baumannii ΔlpxO mutant (ΔlpxO), and A. baumannii ΔlpxO::Tn7lpxO mutant (ΔlpxO::Tn7lpxO) by reverse transcriptase quantitative real‐time PCR. Values are presented as the means ± SD from three independent cDNA preparations measured in duplicate. (B) tnfα expression in il10−/− iBMDMs infected for 5 h with UV-killed A. baumannii ATCC 17978, A. baumannii ΔlpxO mutant (ΔlpxO), and A. baumannii ΔlpxO::Tn7lpxO mutant (ΔlpxO::Tn7lpxO) by reverse transcriptase quantitative real‐time PCR. Values are presented as the means ± SD from two independent cDNA preparations measured in duplicate. (C) il10 expression in control (AS) and CREB siRNA-transfected iBMDMs infected for 5 h with UV-killed A. baumannii ATCC 17978 by reverse transcriptase quantitative real‐time PCR. Values are presented as the means ± SD from two independent cDNA preparations measured in duplicate. (D) Immunoblot analysis of phosphor-CREB (pCREB) and tubulin levels in lysates of iBMDMs infected with UV-killed A. baumannii ATCC 17978, A. baumannii ΔlpxO mutant (ΔlpxO), and A. baumannii ΔlpxO::Tn7lpxO mutant (ΔlpxO::Tn7lpxO). Data are representative of at least three independent experiments. In panels A to C, P values were <0.05 (*) and <0.0001 (****) versus A. baumannii ATCC 17978, determined using one-way ANOVA with Bonferroni contrasts. n.s., not significant.