TABLE 1.
Strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype or comment(s) | Source or reference |
|---|---|---|
| Strains | ||
| A. baumannii | ||
| ATCC 17978 | Wild-type strain | American Type Culture Collection |
| ATCC 17978-ΔlpxO | ATCC 17978 with the lpxO gene inactivated | This study |
| ATCC 17978-ΔlpxO::Tn7lpxO | ATCC 17978, ΔlpxO; Tn7Km_17978lpxOCom integrated into attTn7 site, Kmr | This study |
| E. coli | ||
| C600 | thi thr leuB tonA lacY supE | Laboratory collection |
| GT115 | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 rpsL (strA) endA1 Δdcm uidA(ΔmluI)::pir-116 ΔsbcC-sbcD | InvivoGen |
| β2163 | F RP4-2-Tc::Mu-d dapA::(erm-pir), Kmr, Emr | 43 |
| MG1655 | F− λ− ilvG rfb-50 rph-1 | Laboratory collection |
| BN1 | W3110 ΔeptA ΔlpxT ΔpagP | 19 |
| BN1 ΔlpxL | BN1 ΔlpxL::FRT; the lpxL gene was inactivated | 40 |
| BN1 ΔlpxL::Tn7lpxO | BN1 ΔlpxL::FRT; Tn7Km_17978lpxOCom integrated into attTn7 site, Kmr | This study |
| Plasmids | ||
| pGEM-T Easy | Cloning plasmid, Ampr | Promega |
| pGPI-SceI-2 | Suicide vector, R6Kgamma origin of replicaion, Mob−, carries an I-SceI endonuclease site, Tpr | 42 |
| pDAI-SceI-SacB | Expresses the I-SceI endonuclease gene, sacB, Tetr | 42 |
| pTNS2 | T7 transposase expression vector, oriR6K, Ampr | 49 |
| pUC18R6kT-mini-Tn7TKm | pUC18R6KT-mini-Tn7TKm complementation vector, Ampr, Kmr | 44 |
| pGEMΔlpxO | pGEM-T Easy containing ΔlpxO; Ampr | This study |
| pGPI-SceiI-2ΔlpxO | pGPI-SceI-2 containing ΔlpxO, Tpr | This study |
| pUC18R6kT-mini-Tn7TKm-17978lpxO | pUC18R6kT-mini-Tn7TKm containing lpxO gene for complementation, Ampr, Kmr | This study |