FIG 7.

Decreased enterotoxin digestion under addition of PGM. Supernatant of strain F837/76 was incubated with PBS, 0.25% PGM in PBS, 1 μg/μl trypsin, pancreatin, chymotrypsin or pepsin, or a mixture of the enzymes and 0.25% PGM in PBS for 2 h at 37°C. Proteinase K was used at concentrations of 0.1 and 0.01 μg/μl, and the incubation time was 15 min. Subsequently, Western blot analyses specific for NheB were performed.