TABLE 1.
Genes of interest differentially expressed in B. cereus strain F837/76a
| Gene | Fold change | P value | Gene product | COG | qPCR |
|---|---|---|---|---|---|
| Upregulated | |||||
| bcf_04565 | 534.8 | 5.00E−05 | S-layer protein | No | ✓ |
| bcf_04560 | 469.2 | 5.00E−05 | S-layer protein | No | ✓ |
| bcf_27370 | 462.9 | 5.00E−05 | Hemolysin III-like protein | U | ✓ |
| bcf_09850 | 252.4 | 5.00E−05 | Serine protease | O | ✓ |
| bcf_16240 | 221.2 | 5.00E−05 | Internalin | No | ✓ |
| bcf_08380 | 214.5 | 5.00E−05 | Flagellin protein FlaA | N | ✓ |
| bcf_13835 | 207.8 | 5.00E−05 | Chitin binding protein | R | ✓ |
| bcf_05660 | 200.3 | 5.00E−05 | S-layer protein | No | ✓ |
| bcf_17385 | 195.0 | 5.00E−05 | Collagenase | S | ✓ |
| bcf_26895 | 193.5 | 5.00E−05 | Collagen adhesion protein | S | ND |
| bcf_17575 | 192.2 | 5.00E−05 | Internalin | No | ✘ |
| bcf_01910 | 170.5 | 5.00E−05 | Zinc metalloprotease | R | ✓ |
| bcf_24405 | 161.7 | 0.01445 | Cell envelope-bound metalloprotease | No | ✓ |
| bcf_24230 | 154.5 | 5.00E−05 | Bacitracin export permease protein BceB like protein | V | ✓ |
| bcf_17530 | 141.8 | 5.00E−05 | Cell wall endopeptidase, family M23/M37 | M | ✓ |
| bcf_19505 | 138.7 | 5.00E−05 | Lon-like protease with PDZ domain | T | ND |
| bcf_18185 | 138.4 | 5.00E−05 | Internalin | S | ✘ |
| bcf_17340 | 117.9 | 5.00E−05 | Bile acid sodium symporter | R | ✓ |
| bcf_15285 | 109.6 | 0.00035 | HblB protein | No | ✘ |
| bcf_00530 | 109.3 | 0.00115 | ATP-dependent Clp protease, ATP-binding subunit ClpC | O | ✓ |
| bcf_09265 | 100.4 | 0.00335 | Putative nonhemolytic enterotoxin lytic component L1 (NheB) | No | ✓ |
| bcf_13655 | 15.0 | 5.00E−05 | ATP-dependent Clp protease proteolytic subunit | O | ND |
| bcf_08015 | 14.4 | 5.00E−05 | Internalin | No | ✘ |
| Downregulated | |||||
| bcf_03465 | 182.5 | 5.00E−05 | Broad-substrate-range phospholipase C | No | ✓ |
| bcf_04545 | 159.9 | 5.00E−05 | Protein export cytoplasm protein SecA ATPase RNA helicase | U | ✓ |
| bcf_16685 | 154.2 | 5.00E−05 | Hemolysin-like protein containing CBS domains | R | ✓ |
| bcf_15280 | 126.1 | 0.0001 | Hemolysin BL binding component precursor | No | ✓ |
| bcf_13360 | 107.9 | 0.00045 | Bacillolysin | O | ✓ |
| bcf_18650 | 106.3 | 5.00E−05 | Phosphatidylinositol-specific phospholipase C | No | ✓ |
a
Measurements were taken after 3 h of incubation in mMOD minimal medium supplemented with 0.25% PGM. Genes are sorted according to their fold change in expression. Allocation to the COG categories is shown (27, 28), as is the confirmation of differential expression by qRT-PCR. ✓, differential expression confirmed by qRT-PCR. ✘, differential expression not confirmed by qRT-PCR. ND, not determined.