TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid identifier | Description | Source or reference |
|---|---|---|
| S. Typhimurium strains | ||
| IR715 | Nalidixic acid-resistant strain of Salmonella enterica serovar Typhimurium 14028S | 53 |
| AJB715 | IR715 phoN::Kanr | 54 |
| CG6 | IR715 ΔlldD | 19 |
| CG124 | IR715 ΔcydA | 19 |
| CG200 | IR715 ΔlldR | This study |
| CG226 | IR715 ΔlldP | This study |
| CG254 | IR715 lldD::pCG254 | This study |
| CG267 | IR715 ΔarcA lldD::pCG254 | This study |
| CG268 | IR715 ΔarcB lldD::pCG254 | This study |
| CG270 | IR715 ΔcydA lldD::pCG254 | This study |
| MW118 | IR715 ΔarcB | This study |
| MW119 | IR715 ΔarcA | This study |
| E. coli strains | ||
| DH5α λpir | F− endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 φ80dlacZΔM15 λpir | 55 |
| S17-1 λpir | zxx::RP4 2-(Tetr::Mu) (Kanr::Tn7) λpir | 56 |
| Plasmids | ||
| pRDH10 | ori(R6K) mobRP4 Cmr Tetr sacRB | 54 |
| pFUSE | ori(R6K) mobRP4 Cmr lacZYA | 57 |
| pCG226 | Upstream and downstream regions of S. Typhimurium lldP in pRDH10 | This study |
| pCG254 | Fragment of lldD in pFUSE | This study |
| pCG200 | Upstream and downstream regions of S. Typhimurium lldR in pRDH10 | This study |
| pMW195 | Upstream and downstream regions of S. Typhimurium arcA in pRDH10 | This study |
| pMW89 | Upstream and downstream regions of S. Typhimurium arcB in pRDH10 | This study |