TABLE 2.
Primers and RT-qPCR parameters
| Target | Sequences | Limit of quantification (CT value) | Linear dynamic range (no. of copies/μl) | Source or reference |
|---|---|---|---|---|
| lldP | 5′-TGCTGGCGTTCGCGTTTATC-3′, 5′-CTCTCTACAGGCTACCGCGG-3′ | 38 | 2.69 × 108–2.7 | This study |
| lldR | 5′-ACCGCTGCCGACAAAGAGAA-3′, 5′-CTTTCATCTGGCGATCGCGG-3′ | 37 | 2.69 × 108–2.7 | This study |
| lldD | 5′-TCCGTAACGGGCTGGATGTC-3′, 5′-GAAGGTCGCCATGACCCTGA-3′ | 33 | 2.69 × 108–2.7 | This study |
| dld | 5′-CCGGAGCAGATCCTGAGCAA-3′, 5′-TTTGAATCTTCCGGCTGCGC-3′ | 37 | 2.10 × 108–2.1 | This study |
| gmk | 5′-TTGGCAGGGAGGCGTTT-3′, 5′-GCGCGAAGTGCCGTAGTAAT-3′ | 34 | 2.27 × 109–22.7 | 58 |
| 16S rRNA | 5′-CAGAAGAAGCACCGGCTAACTC-3′, 5′-GCGCTTTACGCCCAGTAATT-3′ | 33 | 7.21 × 107–72.1 | 58 |
| Cxcl1 | 5′-TGCACCCAAACCGAAGTCAT-3′, 5′-TTGTCAGAAGCCAGCGTTCAC-3′ | 32 | 4.86 × 107–48.6 | 59 |
| Nos2 | 5′-TTGGGTCTTGTTCACTCCACGG-3′, 5′-CCTCTTTCAGGTCACTTTGGTAGG-3′ | 37 | 1.04 × 108–1.04 × 102 | 60 |
| Tnf | 5′-AGCCAGGAGGGAGAACAGAAAC-3′, 5′-CCAGTGAGTGAAAGGGACAGAACC-3′ | 40 | 3.2 × 107–32 | 61 |
| Gapdh | 5′-TGTAGACCATGTAGTTGAGGTCA-3′, 5′-AGGTCGGTGTGAACGGATTTG-3′ | 40 | 2.31 × 108–2.31 × 102 | 59 |