FIG 4.

Coinfection of BMDM with WT and ΔtolC Schu S4. BMDM were infected singly with either WT Schu S4 or the ΔtolC mutant at an MOI of 500 or coinfected with the indicated MOI of WT Schu S4 and the ΔtolC mutant. At 24 h postinfection, culture supernatants were collected and assayed for the levels of LDH release. Data represent means ± SEMs from at least three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; calculated by one-way ANOVA with Tukey’s multiple-comparison posttest.