FIG 6.

Pulmonary responses during disease phase are primarily MyD88 independent. Groups of 5 to 10 C57BL/6 or Myd88^−/− mice were challenged by intranasal infection with 2,000 CFU of Y. pestis CO92. On day 3 postinfection, mice were euthanized, blood was collected, lungs were removed and homogenized in sterile PBS, and serum and lung homogenates were analyzed for the following cytokines by a Luminex multiplex assay: IL-1β (A), IL-6 (B), IL-10 (C), TNF-α (D), RANTES (E), and IFN-γ (F). n = 20 (B and D to F) or n = 10 (A and C) mice per group, with data collected in 2 to 4 independent trials. Bars represent standard errors. Data from all trials were combined and analyzed by the Mann-Whitney test. ****, P < 0.0001; NS, not significant.