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Effects of S. aureus biofilm-conditioned medium on PRR-mediated expression of iNOS mRNA in RAW 264.7 macrophages. RAW 264.7 cells were treated with normal culture medium (Control) or BIO-CM in the presence or absence of the PRR agonists Pam2CSK4 (Pam2) (agonist for TLR2), CpG (agonist for TLR9), and MDP (agonist for NOD2). Treatment was conducted for 8 h, and the expression of iNOS mRNA was quantified by qPCR analysis (n = 4). *, P < 0.05; **, P < 0.01.
