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. 2019 Mar 25;87(4):e00643-18. doi: 10.1128/IAI.00643-18

FIG 4.

FIG 4

Effects of S. aureus biofilm-conditioned medium on expression of KLF2 in RAW 264.7 macrophages. (A) qPCR analysis of expression of KLF2 mRNA from RAW 264.7 cells treated with PLK-CM or BIO-CM for 2 or 8 h (n = 6 per group). *, P < 0.05. (B) Representative Western blot images of expression of the KLF2 protein from RAW 264.7 cells treated with PLK-CM or BIO-CM for 2 h. β-Actin expression was used as an internal control. (C) Fluorescence images of RAW 264.7 cells expressing KLF2 protein. Cells were treated with PLK-CM or BIO-CM for 30 min and then immunostained with anti-KLF2 antibody (green). Bar = 40 μm.